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S100A8 antibody

Anti-S100A8 antibody used in Western Blot (WB). GTX54721
Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX54721
Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX54721

Cat. No. GTX54721

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all S100A8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-93 of human S100A8 (NP_002955.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A8 , 60B8AG , CAGA , CFAG , CGLA , CP-10 , L1Ag , MA387 , MIF , MRP8 , NIF , P8

Cellular Localization

Secreted,Cytoplasm,Cytoplasm, cytoskeleton

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-S100A8 antibody used in Western Blot (WB). GTX54721

GTX54721 WB Image

WB analysis of various sample lysates using GTX54721 S100A8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX54721

GTX54721 IHC-P Image

IHC-P analysis of human skin cancer tissue using GTX54721 S100A8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX54721

GTX54721 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX54721 S100A8 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Kato R et al. Drug Metab Dispos 2019; The 2-Hydroxyiminostilbene Metabolite of Carbamazepine or the Supernatant from Incubation of Hepatocytes with Carbamazepine Activates Inflammasomes; Implications for Carbamazepine-induced Hypersensitivity Reactions.
Application : WB
Reactivity : Human
Besold AN et al. Metallomics 2018; 10 (12) : 1728-1742 Antimicrobial action of calprotectin that does not involve metal withholding.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399