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SA2 antibody

Anti-SA2 antibody used in Western Blot (WB). GTX130299
Anti-SA2 antibody used in Western Blot (WB). GTX130299
Anti-SA2 antibody used in Immunoprecipitation (IP). GTX130299
Anti-SA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130299

Cat. No. GTX130299

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stromal antigen 2 , SA-2 , SA2 , SCC3B , bA517O1.1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-SA2 antibody used in Western Blot (WB). GTX130299

GTX130299 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:6000.

Anti-SA2 antibody used in Western Blot (WB). GTX130299

GTX130299 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA2 antibody (GTX130299) diluted at 1:1000.

Anti-SA2 antibody used in Immunoprecipitation (IP). GTX130299

GTX130299 IP Image

Immunoprecipitation of SA2 protein from HeLa whole cell extracts using 5 μg of SA2 antibody (GTX130299).
Western blot analysis was performed using SA2 antibody (GTX130299).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130299

GTX130299 ICC/IF Image

SA2 antibody detects SA2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SA2 protein stained by SA2 antibody (GTX130299) diluted at 1:500.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

REFERENCE

There are currently no references for SA2 antibody (GTX130299). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169