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SAFB2 antibody [N2C1], Internal

Anti-SAFB2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115272
Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272
Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272
Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272
Anti-SAFB2 antibody [N2C1], Internal used in Western Blot (WB). GTX115272

Cat. No. GTX115272

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SAFB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SAFB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619726

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scaffold attachment factor B2

Background

Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Database

Research Area

DATA IMAGES

Anti-SAFB2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115272

GTX115272 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Scaffold attachment factor B2(GTX115272) antibody at 1:200 dilution.

Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272

GTX115272 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Scaffold attachment factor B2(GTX115272) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272

GTX115272 IHC-P Image

SAFB2 antibody [N2C1], Internal detects SAFB2 protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SAFB2 antibody [N2C1], Internal (GTX115272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAFB2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX115272

GTX115272 IHC-P Image

SAFB2 antibody [N2C1], Internal detects SAFB2 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAFB2 antibody [N2C1], Internal (GTX115272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAFB2 antibody [N2C1], Internal used in Western Blot (WB). GTX115272

GTX115272 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX115272 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169