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SEC23A antibody [N1C1]

Anti-SEC23A antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109488
Anti-SEC23A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109488
Anti-SEC23A antibody [N1C1] used in Western Blot (WB). GTX109488
Anti-SEC23A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109488

Cat. No. GTX109488

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SEC23A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEC23A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951838

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sec23 homolog A, coat complex II component , CLSD , hSec23A

Cellular Localization

Smooth endoplasmic reticulum membrane; Peripheral membrane protein , Golgi apparatus membrane; Peripheral membrane protein (Potential)

Background

The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEC23A antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109488

GTX109488 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SEC23A(GTX109488) antibody at 1:200 dilution.

Anti-SEC23A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109488

GTX109488 IHC-P Image

SEC23A antibody [N1C1] detects SEC23A protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEC23A antibody [N1C1] (GTX109488) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEC23A antibody [N1C1] used in Western Blot (WB). GTX109488

GTX109488 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109488 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEC23A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109488

GTX109488 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC23A(GTX109488) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kondo Y et al. JCI Insight 2018; 3 (14) Site-1 protease deficiency causes human skeletal dysplasia due to defective inter-organelle protein trafficking.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169