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SFPQ antibody

Anti-SFPQ antibody used in Western Blot (WB). GTX112116
Anti-SFPQ antibody used in IHC (Paraffin sections) (IHC-P). GTX112116

Cat. No. GTX112116

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SFPQ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SFPQ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168247

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor proline and glutamine rich , POMP100 , PPP1R140 , PSF

Cellular Localization

Nucleus matrix

Database

Research Area

DATA IMAGES

Anti-SFPQ antibody used in Western Blot (WB). GTX112116

GTX112116 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
5% SDS PAGE
GTX112116 diluted at 1:10000

Anti-SFPQ antibody used in IHC (Paraffin sections) (IHC-P). GTX112116

GTX112116 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using SFPQ(GTX112116) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169