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SHC1 (phospho Tyr239) antibody

Anti-SHC1 (phospho Tyr239) antibody used in Western Blot (WB). GTX54986
Anti-SHC1 (phospho Tyr239) antibody used in IHC (Paraffin sections) (IHC-P). GTX54986

Cat. No. GTX54986

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all SHC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Product Note

This antibody is raised against human SHC1 phosphorylated at Tyr349 of p66Shc (Uniprot #P29353-1). Based on sequence homology, it is corresponding to human SHC1 phosphorylated at Tyr239 of p52Shc (Uniprot #P29353-2).

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of SHC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SHC adaptor protein 1 , SHC , SHCA

Cellular Localization

Cytoplasm

Background

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-SHC1 (phospho Tyr239) antibody used in Western Blot (WB). GTX54986

GTX54986 WB Image

WB analysis of EGF-treated HeLa (A), EGF-treated HUVEC(B) whole cell lysates using GTX54986 SHC1 (phospho Tyr239) antibody.

Anti-SHC1 (phospho Tyr239) antibody used in IHC (Paraffin sections) (IHC-P). GTX54986

GTX54986 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX54986 SHC1 (phospho Tyr239) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399