GeneTex
United States (US)

SHP2 (phospho Tyr542) antibody

GTX32349_20190131_WB_19013102_374.jpg
GTX32349_20190131_IHC-P_19013102_826.jpg

Cat No. GTX32349

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of SHP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Non-Receptor Type 11,Bptp3,Cfc,Jmml,Metcds,Ns1,Ptp-1D,Ptp2C,Sh-Ptp2,Sh-Ptp3,Shp2,Ptpn11

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES
GTX32349_20190131_WB_19013102_374.jpg

GTX32349 WB Image

WB analysis of A549 (A), HeLa (B), Raw264.7 (C) whole cell lysates using GTX32349 SHP2 (phospho Tyr542) antibody.

GTX32349_20190131_IHC-P_19013102_826.jpg

GTX32349 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32349 SHP2 (phospho Tyr542) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE
Package List Price ($)
$ 319