Recommended Starting Dilutions:
For ELISA: Use at a concentration of 0.1-1.0ug/mL.
For IP : Use at a concentration of 5-10ug/ml.
For WB: USe at a concentration of 1-3ug/ml.
Optimal dilutions/concentrations should be determined by the end user.
HeLa cervical carcinoma, Jurkat leukemia T cell and Wi-38 lung broblast cell lysates
Recognizes human SKP1 (p19) protein at 19kD
PBS, pH 7.4 contianing 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant human SKP1 (p19) protein
Protein A affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
S-Phase Kinase Associated Protein 1,Emc19,Ocp-Ii,Ocp2,Skp1A,Tceb1L,P19A,Skp1
Skp1 is an F-box protein which functions as a substrate recognition component of the SCF ubiquitin ligase complex. It binds to cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin proteolysis through an F-box motif. It is also involved in the control of beta-catenin levels the activity of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor.