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SLBP antibody

KO/KD Orthogonal
Anti-SLBP antibody used in Western Blot (WB). GTX130597
Anti-SLBP antibody used in Western Blot (WB). GTX130597
Anti-SLBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130597

Cat. No. GTX130597

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SLBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

HepG2 membrane fraction extract , 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886306

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

stem-loop binding protein , HBP

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLBP antibody used in Western Blot (WB). GTX130597

GTX130597 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SLBP antibody (GTX130597) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLBP antibody used in Western Blot (WB). GTX130597

GTX130597 WB Image

Orthogonal

HepG2 whole cell and cytoplasm extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLBP antibody (GTX130597) diluted at 1:1000.

Anti-SLBP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130597

GTX130597 ICC/IF Image

SLBP antibody detects SLBP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLBP protein stained by SLBP antibody (GTX130597) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for SLBP antibody (GTX130597). Be the first to share your publications with this product.
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REVIEW

SLBP antibody Cat. No. GTX130597

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-SLBP antibody used in Western Blot (WB). GTX130597
Date : Anonymous submitted on 21-Jul-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7, T47D, HeLa, HCT116, HT29, 293T cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:3000, 4°C, 14Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169