APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , H1299 , HeLa , HepG2
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLC25A22. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951927
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 25 member 22 , EIEE3 , GC-1 , GC1 , NET44
Cellular Localization
Mitochondrion inner membrane; Multi-pass membrane protein
Background
The SLC25 gene family encodes mitochondrial carriers that transport a variety of metabolites across the inner mitochondrial membrane (Palmieri, 2004 [PubMed 14598172]). SLC25A22, also known as GC1, is 1 of the 2 mitochondrial glutamate/H+ symporters, the other being SLC25A18 (MIM 609303).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700](/templates/default/js/lightbox/images/loading.gif "Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700")
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GTX110700 WB Image
Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX110700 diluted at 1:10000
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GTX110700 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using SLC25A22(GTX110700) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110700 ICC/IF Image
SLC25A22 antibody [N1C3] detects SLC25A22 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A22 protein stained by SLC25A22 antibody [N1C3] (GTX110700) diluted at 1:200.
Blue: Hoechst 33342 staining.
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GTX110700 IHC-P Image
SLC25A22 antibody [N1C3] detects SLC25A22 protein at mitochondria in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SLC25A22 antibody [N1C3] (GTX110700) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110700 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
10% SDS PAGE
GTX110700 diluted at 1:1000
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REFERENCE
There are currently no references for SLC25A22 antibody [N1C3] (GTX110700). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SLC25A22 antibody [N1C3] (GTX110700). Be the first to share your experience with this product.
![Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700](/upload/website/prouct_img/small/GTX110700/GTX110700_40107_WB_M_Brain_w_23060500_362.webp "Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700](/upload/website/prouct_img/small/GTX110700/GTX110700_40107_IHC_w_23060500_580.webp "Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110700](/upload/website/prouct_img/small/GTX110700/GTX110700_40107_20160810_IFA_w_23060500_705.webp "Anti-SLC25A22 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700](/upload/website/prouct_img/small/GTX110700/GTX110700_40107_20151227_IHC-P_w_23060500_592.webp "Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700](/upload/website/prouct_img/small/GTX110700/GTX110700_WB_w_23060500_629.webp "Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700](/upload/website/prouct_img/normal/GTX110700/GTX110700_40107_WB_M_Brain_w_23060500_362.webp "Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700](/upload/website/prouct_img/normal/GTX110700/GTX110700_40107_IHC_w_23060500_580.webp "Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110700](/upload/website/prouct_img/normal/GTX110700/GTX110700_40107_20160810_IFA_w_23060500_705.webp "Anti-SLC25A22 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700](/upload/website/prouct_img/normal/GTX110700/GTX110700_40107_20151227_IHC-P_w_23060500_592.webp "Anti-SLC25A22 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110700")
![Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700](/upload/website/prouct_img/normal/GTX110700/GTX110700_WB_w_23060500_629.webp "Anti-SLC25A22 antibody [N1C3] used in Western Blot (WB). GTX110700")