APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Sheep, Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SLC25A6. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11169465
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
solute carrier family 25 member 6 , AAC3 , ANT , ANT 2 , ANT 3 , ANT3 , ANT3Y
Cellular Localization
Mitochondrion inner membrane
Background
This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the mitochondrial matrix into the cytoplasm. The protein is implicated in the function of the permability transition pore complex (PTPC), which regulates the release of mitochondrial products that induce apoptosis. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110983 ICC/IF Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX110983 IHC-P Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110983 IHC-P Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110983 IHC-P Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
SLC25A6 antibody (GTX110983) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110983 ICC/IF Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SLC25A6 protein stained by SLC25A6 antibody (GTX110983) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX110983 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A6 antibody (GTX110983) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110983 IP Image
Immunoprecipitation of SLC25A6 protein from 293T whole cell extracts using 5 μg of SLC25A6 antibody (GTX110983).
Western blot analysis was performed using SLC25A6 antibody (GTX110983).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX110983 IHC-P Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat muscle.SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110983 IHC-P Image
SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (GTX110983) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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