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SLK antibody

KO/KD
Anti-SLK antibody used in Western Blot (WB). GTX103840
Anti-SLK antibody used in IHC (Paraffin sections) (IHC-P). GTX103840
Anti-SLK antibody used in Western Blot (WB). GTX103840

Cat. No. GTX103840

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SLK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7 , 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163041

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

STE20 like kinase , LOSK , STK2 , bA16H23.1 , se20-9

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-SLK antibody used in Western Blot (WB). GTX103840

GTX103840 WB Image

KO/KD

Wild-type (WT) and SLK knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLK antibody (GTX103840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLK antibody used in IHC (Paraffin sections) (IHC-P). GTX103840

GTX103840 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using SLK(GTX103840) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLK antibody used in Western Blot (WB). GTX103840

GTX103840 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
5% SDS PAGE
GTX103840 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169