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SLUG antibody

Orthogonal
Anti-SLUG antibody used in Western Blot (WB). GTX121924
Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924
Anti-SLUG antibody used in Western Blot (WB). GTX121924

Cat. No. GTX121924

Reference 5
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all SLUG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

NIH-3T3 nuclear extract

Predict Reactivity

Human, Rat, Zebrafish, Sheep, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLUG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10728929

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

snail family transcriptional repressor 2 , SLUG , SLUGH , SLUGH1 , SNAIL2 , WS2D

Cellular Localization

Nucleus

Background

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SLUG antibody used in Western Blot (WB). GTX121924

GTX121924 WB Image

NIH-3T3 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SLUG antibody (GTX121924) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SLUG antibody used in IHC (Paraffin sections) (IHC-P). GTX121924

GTX121924 IHC-P Image

SLUG antibody detects SLUG protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
SLUG antibody (GTX121924) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SLUG antibody used in Western Blot (WB). GTX121924

GTX121924 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Soyama H et al. J Obstet Gynaecol Res 2020; (Epub) Placenta previa may acquire invasive nature by factors associated with epithelial-mesenchymal transition and matrix metalloproteinases.
Application : IHC-P
Liu YH et al. Sci Rep 2017; 7 : 41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Cheng M et al. Clin Exp Gastroenterol 2015; 8 : 13-29 Cathelicidin suppresses colon cancer development by inhibition of cancer associated fibroblasts.
Kuo TC et al. J Clin Invest 2013; 123 (3) : 1082-95 Angiopoietin-like protein 1 suppresses SLUG to inhibit cancer cell motility.
Application : IHC
Submit a Reference

REVIEW

SLUG antibody Cat. No. GTX121924

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SLUG antibody used in Western Blot (WB). GTX121924
Date : Anonymous submitted on 08-Aug-2022
Score :
Application Tested : WB
Sample : Huh7 cells
Amount used : 30μg
Blocking : 5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169