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SMYD1 antibody

Anti-SMYD1 antibody used in Western Blot (WB). GTX119484
Anti-SMYD1 antibody used in Western Blot (WB). GTX119484
Anti-SMYD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119484
Anti-SMYD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119484
Anti-SMYD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119484

Cat. No. GTX119484

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SMYD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , mouse heart

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMYD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162404

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET and MYND domain containing 1 , BOP , KMT3D , ZMYND18 , ZMYND22

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.

Database

Research Area

DATA IMAGES

Anti-SMYD1 antibody used in Western Blot (WB). GTX119484

GTX119484 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
7.5% SDS PAGE
GTX119484 diluted at 1:10000

Anti-SMYD1 antibody used in Western Blot (WB). GTX119484

GTX119484 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX119484 diluted at 1:10000

Anti-SMYD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119484

GTX119484 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMYD1(GTX119484) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SMYD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119484

GTX119484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using SMYD1 antibody(GTX119484) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SMYD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX119484

GTX119484 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using SMYD1 antibody(GTX119484) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SMYD1 antibody (GTX119484). Be the first to share your publications with this product.
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REVIEW

SMYD1 antibody Cat. No. GTX119484

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-SMYD1 antibody used in Western Blot (WB). GTX119484
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5% milk in TBST
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-SMYD1 antibody used in Western Blot (WB). GTX119484
Date : Anonymous submitted on 31-Aug-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : RD cells
Blocking : 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Luc, Lane 2: SMYD1 knockdown clone 1,Lane 3: SMYD1 knockdown clone 2, Lane 4: RD cell lysate
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169