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SNAI1 antibody

Comparison Orthogonal Overexpression
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

Cat. No. GTX100754

Reference 25
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SNAI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa (starvation for 16 hr 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min) , SNAI1-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618674

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

Cellular Localization

Nucleus

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX100754) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Orthogonal

Untreated (–) and treated (+) Hela whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

SNAI1 antibody detects SNAI1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

GTX100754 ICC/IF Image

Orthogonal

SNAI1 antibody detects SNAI1 protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 stained by SNAI1 antibody (GTX100754) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100754

GTX100754 IHC-P Image

SNAI1 antibody detects SNAI1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SNAI1 stained by SNAI1 antibody (GTX100754) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

GTX100754 ICC/IF Image

The data was published in the journal Oncotarget in 2017.PMID: 27494900

REFERENCE

Application Reference

Wang CW et al. Front Immunol 2023; 14 : 1225348 Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models.
Chiou HC et al. Antioxidants (Basel) 2023; 12 (2) Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human.
Application : WB
Reactivity : Human
Kano H et al. Cancer Sci 2023; 114 (4) : 1479-1490 Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis.
Application : IHC-P
Reactivity : Human
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10 : 809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Application : ICC/IF
Reactivity : Human
Li Xiao et al. Int J Mol Sci 2021; 22 (19) LEF1 Enhances the Progression of Colonic Adenocarcinoma via Remodeling the Cell Motility Associated Structures.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

SNAI1 antibody Cat. No. GTX100754

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Date : Anonymous submitted on 03-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : TW2.6 cells
Amount used : 15μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 1Hr
Submit a Review
Application Reference
Wang CW et al. Front Immunol 2023; 14:1225348 Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models.
Chiou HC et al. Antioxidants (Basel) 2023; 12 (2) Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human.
Application : WB
Reactivity : Human
Kano H et al. Cancer Sci 2023; 114 (4):1479-1490 Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis.
Application : IHC-P
Reactivity : Human
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10:809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
Application : ICC/IF
Reactivity : Human
Li Xiao et al. Int J Mol Sci 2021; 22 (19) LEF1 Enhances the Progression of Colonic Adenocarcinoma via Remodeling the Cell Motility Associated Structures.
Application : WB
Reactivity : Human
Brzozowa-Zasada M et al. Prz Gastroenterol 2021; 16 (4):339-345 Immunohistochemical expression of Snail1 protein in colorectal adenocarcinoma samples and its prognostic activity in Caucasian patients.
Application : IHC-P
Reactivity : Human
Chung HH et al. Int J Mol Sci 2020; 21 (14) NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer.
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Xiao L et al. Life Sci 2020; 253:117740 Roles of N-terminal Annexin A2 phosphorylation sites and miR-206 in colonic adenocarcinoma.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
Application : WB
Reactivity : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.
Application : WB
Reactivity : Human
Roth B et al. Oncotarget 2017; 8 (21):34205-34222 Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis.
Application : ICC/IF
Reactivity : Mouse
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Hsu LS et al. Int J Med Sci 2017; 14 (6):530-535 KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities.
Application : WB
Reactivity : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Li HJ et al. Oncogene 2016; 35 (17):2266-78 NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
Yang HL et al. Biochim Biophys Acta 2016; 1864 (1):151-168 Lucidone Promotes the Cutaneous Wound Healing Process via Activation of the PI3K/AKT, Wnt/β-catenin and NF-κB Signaling Pathways.
Application : WB
Reactivity : Human
Wang CY et al. CLINICS ONCOL 2016; 1:1015 Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with Down-Regulation of SNAIL-1 and Induction of Let-7.
Application : WB
Reactivity : Human
Wang TH et al. Oncotarget 2015; 6 (27):23342-57 Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Agliarulo I et al. Biochim Biophys Acta 2015; 1853 (10 Pt A):244958 TRAP1 controls cell migration of cancer cells in metabolic stress conditions: Correlations with AKT/p70S6K pathways.
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
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