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SNAI1 antibody

KO/KD Comparison Orthogonal Overexpression
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

Cat. No. GTX125918

Reference 26

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SNAI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Hela(1 ng/ml TGF-beta and 300 ng/ml of EGF for 2 hr) , HeLa (starvation for 16 hr 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min) , SK-N-SH , IMR-32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

Cellular Localization

Nucleus , Cytoplasm

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX125918) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

KO/KD

Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125918

GTX125918 ICC/IF Image

SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 protein stained by SNAI1 antibody (GTX125918) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918

GTX125918 IHC-P Image

SNAI1 antibody detects SNAI1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SNAI1 antibody (GTX125918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918

GTX125918 IHC-P Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

REFERENCE

Application Reference

TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
Application : WB
Wu WS et al. Am J Cancer Res 2023; 13 (10) : 4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
TH Hung et al. Biomed J 2023; : 100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
Application : WB
Reactivity : Human
Wang L et al. Bioengineered 2022; 13 (5) : 12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
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REVIEW

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Application Reference
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
Application : WB
Wu WS et al. Am J Cancer Res 2023; 13 (10):4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
TH Hung et al. Biomed J 2023;:100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
Application : WB
Reactivity : Human
Wang L et al. Bioengineered 2022; 13 (5):12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
Wang LT et al. Cancer Lett 2021; 520:160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
Application : WB
Reactivity : Mouse
Lv F et al. Eur J Pharmacol 2021; 900:174069 Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway.
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
Application : WB
Reactivity : Human
Kim BN et al. Sci Rep 2020; 10 (1):10597 TGF-β induced EMT and stemness characteristics are associated with epigenetic regulation in lung cancer.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
Application : WB
Reactivity : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Harada H et al. Oncol Lett 2019; 17 (1):578-586 Carcinosarcoma of the esophagus: A report of 6 cases associated with zinc finger E-box-binding homeobox 1 expression.
Bacci L et al. Int J Mol Sci 2019; 20 (16) H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.
Application : WB
Reactivity : Human
Liu YP et al. Cancer Sci 2018; 109 (10):3105-3114 Human immunodeficiency virus Tat-TIP30 interaction promotes metastasis by enhancing the nuclear translocation of Snail in lung cancer cell lines.
Hiroki Harada et al. Oncology Letters 2018; 17 (1):578-586 Carcinosarcoma of the esophagus: A report of 6 cases associated with zinc finger E-box-binding homeobox 1 expression.
Application : IHC-P
Lai YJ et al. J Cell Physiol 2018; (Epub) CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Zhenzhen L et al. Clinical Science 2018; 132 (21):2339-2355 MKP2 inhibits TGF-β1-induced epithelial-to-mesenchymal transition in renal tubular epithelial cells through a JNK-dependent pathway.
Application : WB
Reactivity : Rat
Micati DJ et al. Andrology 2018; (Epub) Differential expression profiles of conserved Snail transcription factors in the mouse testis.
Application : IHC-P
Reactivity : Mouse
Lee J et al. J Biol Chem 2018; (Epub) Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
Reactivity : Human
Kuo WY et al. Theranostics 2017; 7 (3):647 stat3/nf-κb-regulated lentiviral tk/gcv suicide gene therapy for *-resistant triple-negative breast cancer.
Application : WB
Reactivity : Human
Yin K et al. Oncotarget 2016; 7 (17):24537-48 MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.
Application : WB
Reactivity : Human
Chung IH et al. Oncotarget 2016; 7 (10):11113-26 Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
Application : IHC-P
Reactivity : Human
Ling HH et al. Exp Cell Res 2016; Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Application : WB
Reactivity : Human
Horvay K et al. EMBO J 2015; 34 (10):1319-35 Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium.
Application : IHC
Wai-Theng Lee et al. Food and Chemical Toxicology 2015; 78:33-41 Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κBdependent MMP-9 and epithelial-mesenchymal transition expressions
Application : WB
Reactivity : Human
SDS
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Glycerol.pdf
Proclin.pdf
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$ 399
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