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SNAP25 antibody

Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839
Anti-SNAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839
Anti-SNAP25 antibody used in IHC (Whole mount) (IHC-Wm). GTX113839
Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839

Cat. No. GTX113839

Reference 16

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SNAP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

rat brain , Mouse brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAP25. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038011

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptosome associated protein 25 , CMS18 , RIC-4 , RIC4 , SEC9 , SNAP , SNAP-25 , SUP , bA416N4.2 , dJ1068F16.2

Cellular Localization

Cytoplasm , perinuclear region , Cell membrane; Lipid-anchor , Cell junction , synapse , synaptosome

Background

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839

GTX113839 IHC-Fr Image

SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SNAP25 antibody used in Western Blot (WB). GTX113839

GTX113839 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113839

GTX113839 ICC/IF Image

SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAP25 protein stained by SNAP25 antibody (GTX113839) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SNAP25(GTX113839) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAP25 antibody used in Western Blot (WB). GTX113839

GTX113839 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody (GTX113839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX113839

GTX113839 IHC-P Image

SNAP25 antibody detects SNAP25 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SNAP25 antibody (GTX113839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAP25 antibody used in IHC (Whole mount) (IHC-Wm). GTX113839

GTX113839 IHC-Wm Image

SNAP25 antibody detects SNAP25 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: SNAP25 stained by SNAP25 antibody (GTX113839) diluted at 1:50.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-SNAP25 antibody used in IHC (Frozen sections) (IHC-Fr). GTX113839

GTX113839 IHC-Fr Image

The data was published in the journal Front Neurol in 2014. PMID: 25002857

REFERENCE

Application Reference

Hong CT et al. Elife 2024; 12 Plasma extracellular vesicle synaptic proteins as biomarkers of clinical progression in patients with Parkinson's disease.
Kun Cao et al. Med Sci Monit 2022; 28 : e933978 Activation of α7 Nicotinic Acetylcholine Receptor by its Selective Agonist Improved Learning and Memory of Amyloid Precursor Protein/Presenilin 1 (APP/PS1) Mice via the Nrf2/HO-1 Pathway.
Application : WB
Reactivity : Mouse
Dandan Liang et al. Protein Cell 2021; 12 (7) : 545-556 Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.
Application : IHC-P
Reactivity : Mouse
Abdallah IM et al. Int J Mol Sci 2021; 22 (3) Blood-Brain Barrier Disruption Increases Amyloid-Related Pathology in TgSwDI Mice.
Application : WB
Reactivity : Mouse
Cao K et al. Aging (Albany NY) 2020; 12 (2) : 1792-1807 The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors.
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REVIEW

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Application Reference
Hong CT et al. Elife 2024; 12 Plasma extracellular vesicle synaptic proteins as biomarkers of clinical progression in patients with Parkinson's disease.
Kun Cao et al. Med Sci Monit 2022; 28:e933978 Activation of α7 Nicotinic Acetylcholine Receptor by its Selective Agonist Improved Learning and Memory of Amyloid Precursor Protein/Presenilin 1 (APP/PS1) Mice via the Nrf2/HO-1 Pathway.
Application : WB
Reactivity : Mouse
Dandan Liang et al. Protein Cell 2021; 12 (7):545-556 Sinoatrial node pacemaker cells share dominant biological properties with glutamatergic neurons.
Application : IHC-P
Reactivity : Mouse
Abdallah IM et al. Int J Mol Sci 2021; 22 (3) Blood-Brain Barrier Disruption Increases Amyloid-Related Pathology in TgSwDI Mice.
Application : WB
Reactivity : Mouse
Cao K et al. Aging (Albany NY) 2020; 12 (2):1792-1807 The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors.
Xu Y et al. Aging (Albany NY) 2020; 12 (14):14205-14218 Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice.
Application : WB
Reactivity : Mouse
Wang XL et al. Aging (Albany NY) 2020; 12 (1):543-570 Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway.
Application : WB
Reactivity : Mouse
Cao K et al. Alzheimers Res Ther 2019; 11 (1):35 Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation.
Al Rihani SB et al. J Alzheimers Dis 2019; (Epub) Granisetron Alleviates Alzheimer's Disease Pathology in TgSwDI Mice Through Calmodulin-Dependent Protein Kinase II/cAMP-Response Element Binding Protein Pathway.
Application : WB
Reactivity : Mouse
Duong QV et al. Int J Mol Sci 2019; 20 (6) Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice.
Application : WB
Reactivity : Mouse
Elfakhri KH et al. Neurobiol Dis 2019; 125:123-134 Multi-faceted therapeutic strategy for treatment of Alzheimer's disease by concurrent administration of * and α-tocopherol.
Application : WB
Reactivity : Mouse
Batarseh YS et al. JNB 2017; (Epub) Oleocanthal-rich extra-virgin olive oil enhances * effect by reducing amyloid-β load and related toxicity in a mouse model of Alzheimer’s disease.
Application : WB
Reactivity : Mouse
Kuzniewska B et al. J Neurosci Methods 2017; 293:226-233 Preparation of polysomal fractions from mouse brain synaptoneurosomes and analysis of polysomal-bound mRNAs.
Application : WB
Reactivity : Mouse
Batarseh YS et al. Neuroscience 2017; (Epub) Oleocanthal ameliorates amyloid-β oligomers' toxicity on astrocytes and neuronal cells: In vitro studies.
Application : WB
Reactivity : Human
Ugun-Klusek A et al. Cell Death Dis 2017; 8 (1):e2531 Continued 26S proteasome dysfunction in mouse brain cortical neurons impairs autophagy and the Keap1-Nrf2 oxidative defence pathway.
Application : WB
Reactivity : Mouse
Koizumi H et al. Front Neurol 2014; 5:98
Application : WB
Reactivity : Rat
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169