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SNF2H antibody

Orthogonal
Anti-SNF2H antibody used in IHC (Paraffin sections) (IHC-P). GTX130794
Anti-SNF2H antibody used in Western Blot (WB). GTX130794
Anti-SNF2H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130794

Cat. No. GTX130794

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all SNF2H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

HepG2 nuclear extract

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SNF2H. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886346

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 , ISWI , SNF2H , WCRF135 , hISWI , hSNF2H

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SNF2H antibody used in IHC (Paraffin sections) (IHC-P). GTX130794

GTX130794 IHC-P Image

SNF2H antibody detects SNF2H protein at nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
SNF2H antibody (GTX130794) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SNF2H antibody used in Western Blot (WB). GTX130794

GTX130794 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SNF2H antibody (GTX130794) diluted at 1:10000.

Anti-SNF2H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130794

GTX130794 ICC/IF Image

SNF2H antibody detects SNF2H protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNF2H protein stained by SNF2H antibody (GTX130794) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169