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SOD1 antibody

KO/KD Orthogonal
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Anti-SOD1 antibody used in Western Blot (WB). GTX100659

Cat. No. GTX100659

Reference 12
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SOD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

Rat brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951972

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100659 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100659) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2018. PMID: 30279365

Anti-SOD1 antibody used in Western Blot (WB). GTX100659

GTX100659 WB Image

The data was published in the journal Oxid Med Cell Longev in 2020.PMID: 32089770

REFERENCE

Application Reference

Ayoubi R et al. F1000Res 2023; 12 : 391 Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1?(SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.
Man-Hau Ho et al. Redox Biol 2021; 46 : 102067 CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.
Application : WB
Reactivity : Mouse
Gentilin E et al. Chem Biol Interact 2021; 345 : 109575 Hydrogen peroxide toxicity on auditory cells: An in vitro study.
Application : WB
Chang HM et al. Antioxidants (Basel) 2021; 10 (5) Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury.
Reactivity : Rat
Murata K et al. J Nat Med 2021; 75 (1) : 142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
Application : IHC-Fr
Reactivity : Mouse
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Submit a Reference

REVIEW

SOD1 antibody Cat. No. GTX100659

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SOD1 antibody used in Western Blot (WB). GTX100659
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and SOD1 KO cells
Amount used : 20μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
Application Reference
Ayoubi R et al. F1000Res 2023; 12:391 Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1?(SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.
Man-Hau Ho et al. Redox Biol 2021; 46:102067 CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury.
Application : WB
Reactivity : Mouse
Gentilin E et al. Chem Biol Interact 2021; 345:109575 Hydrogen peroxide toxicity on auditory cells: An in vitro study.
Application : WB
Chang HM et al. Antioxidants (Basel) 2021; 10 (5) Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury.
Reactivity : Rat
Murata K et al. J Nat Med 2021; 75 (1):142-155 Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice.
Application : IHC-Fr
Reactivity : Mouse
Liu W et al. Oxid Med Cell Longev 2020; 2020:5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Tsai SH et al. FASEB J 2020; 34 (7):9498-9511 Aldehyde dehydrogenase 2 protects against abdominal aortic aneurysm formation by reducing reactive oxygen species, vascular inflammation, and apoptosis of vascular smooth muscle cells.
Application : WB
Reactivity : Human
Wangta Liu et al. Oxidative Medicine and Cellular Longevity 2020; 2020:Article ID 5167292 The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling.
Application : WB
Reactivity : Human
Chou CK et al. Environ Toxicol 2019; 34 (4):401-414 Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes.
Application : WB
Reactivity : Human
Chang YC et al. Int J Mol Sci 2018; 19 (10) Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells.
Application : WB
Reactivity : Human
Chen HW et al. Toxicol Appl Pharmacol 2014; Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats.
Application : WB
Reactivity : Rat
Tury A et al. Dev Neurobiol 2013; Altered expression of atypical PKC and Ryk in the spinal cord of a mouse model of amyotrophic lateral sclerosis.
Application : WB, IHC-Fr
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169