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SOX2 antibody [GT1876]

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

Cat No. GTX627404

Host Mouse
Clonality Monoclonal
Clone Name GT1876
Isotype IgG1
Application WB, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500

Note :

IHC-Fr
The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , human ESC , zebrafish eye

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sry-Box 2,Anop3,Mcops3,Sox2

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404

GTX627404 FACS Image

SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 µg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404

GTX627404 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

REFERENCE
REVIEW

SOX2 antibody [GT1876] Cat No. GTX627404

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black : Isotype control, Green : SOX2 antibody
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169