GeneTex
United States (US)

SOX2 antibody [GT1876]

KO/KD Comparison
GTX627404_40846_20160921_IHC-Fr_18121411_682.jpg
GTX627404_40846_20180119_WB_competitor_watermark_18121411_856.jpg
GTX627404_40846_CT_FACS_18121411_553.jpg
GTX627404_40846_WB_hESC_18121411_889.jpg
GTX627404_40846_20170126_WB_shRNA_watermark_18121411_368.jpg
GTX627404_40846_WB_18121411_293.jpg
GTX627404_40846_WB_Z_eye_18121411_433.jpg
GTX627404_40846_20160824_IHC-Fr_18121411_110.jpg
GTX627404_40846_IP_2_18121411_887.jpg
GTX627404_40846_20150625_IHC_M_18121411_172.jpg

Cat No. GTX627404

Host Mouse
Clonality Monoclonal
Clone Name GT1876
Isotype IgG1
Application WB, IHC-Fr, FACS, IP
Reactivity Human, Mouse, Rat, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500

Note :

IHC-Fr
The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.

Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , human ESC , zebrafish eye

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ANOP3 antibody, MCOPS3 antibody, MGC2413 antibody, SOX2 antibody, transcription factor SOX2 antibody, SRY-related HMG-box gene 2 antibody, transcription factor SOX-2 antibody, SRY (sex determining region Y)-box 2 antibody

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX627404_40846_20160921_IHC-Fr_18121411_682.jpg

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX627404_40846_20180119_WB_competitor_watermark_18121411_856.jpg

GTX627404 WB Image

Comparison

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX627404_40846_CT_FACS_18121411_553.jpg

GTX627404 FACS Image

SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50

GTX627404_40846_WB_hESC_18121411_889.jpg

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

GTX627404_40846_20170126_WB_shRNA_watermark_18121411_368.jpg

GTX627404 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

GTX627404_40846_WB_18121411_293.jpg

GTX627404 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000

GTX627404_40846_WB_Z_eye_18121411_433.jpg

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 µg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500

GTX627404_40846_20160824_IHC-Fr_18121411_110.jpg

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX627404_40846_IP_2_18121411_887.jpg

GTX627404 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627404_40846_20150625_IHC_M_18121411_172.jpg

GTX627404 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

REFERENCE
REVIEW

SOX2 antibody [GT1876] Cat No. GTX627404

Rating ( Average 4 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black : Isotype control, Green : SOX2 antibody
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169