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SOX2 antibody

Anti-SOX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31797
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31797
Anti-SOX2 antibody used in Western Blot (WB). GTX31797
Anti-SOX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31797
Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31797

Cat. No. GTX31797

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SOX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 5 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179) , 3T3/BALB Cell Slide , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX.The immunogen is located within the first 50 amino acids of SOX2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 2 , ANOP3 , MCOPS3

Cellular Localization

Nucleus

Background

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31797

GTX31797 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31797 SOX2 antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31797

GTX31797 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31797 SOX2 antibody.
Working concentration : 20 μg/ml

Anti-SOX2 antibody used in Western Blot (WB). GTX31797

GTX31797 WB Image

WB analysis of 3T3 cell lysate using GTX31797 SOX2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-SOX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31797

GTX31797 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31797 SOX2 antibody.
Working concentration : 5 μg/ml

Anti-SOX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31797

GTX31797 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31797 SOX2 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399