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SOX9 (phospho Ser181) antibody

Anti-SOX9 (phospho Ser181) antibody used in Dot blot (Dot). GTX23696

Cat. No. GTX23696

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot

Reactivity

 Human, Mouse, Bovine
Package
50 μg ($499)
See all SOX9 products

APPLICATION

Application Note

Dot Blot : 1.0 μg/ml
WB : 0.5 to 2.0 μg/ml
Optimal dilutions / concentrations should be determined by the end user.

Calculated MW

56 kDa. ( Note )

Product Note

This antibody reacts predominantly with phosphorylated SOX 9. The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX 9 peptides. It only recognizes the phosphorylated serine 181 of human SOX 9,not other phosphorylated sites or non-phosphorylated SOX 9.

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (QPRRRKpSVKNG)corresponding to human / mouse / bovine SOX 9 at thephosphorylation site of Serine 181.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SRY-box 9 , CMD1 , CMPD1 , SRA1 , SRXX2 , SRXY10

Cellular Localization

Nucleus

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SOX9 (phospho Ser181) antibody used in Dot blot (Dot). GTX23696

GTX23696 Dot Image

Dot blot analysis of the SOX-9 peptide (pSer181) and SOX-9 peptide probed with the anti-SOX-9 (pSer181) (GTX23696) and anti-SOX-9 (GTX23697).
The staining shows that the anti-SOX-9 (pSer181) recognized the SOX-9 peptide (pSer181) only; whole the anti-SOX-9 could bind both the SOX-9 peptide (pSer181) and SOX-9 peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 499