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SPARC antibody

Comparison
Anti-SPARC antibody used in Western Blot (WB). GTX133747
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747
Anti-SPARC antibody used in Western Blot (WB). GTX133747
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747
Anti-SPARC antibody used in Western Blot (WB). GTX133747

Cat. No. GTX133747

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SPARC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SPARC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887090

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted protein acidic and cysteine rich , BM-40 , OI17 , ON

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

Secreted protein acidic and rich in cysteine/osteonectin/BM40, or SPARC, is a matrix-associated protein that elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (ECM) (Bradshaw et al., 2003 [PubMed 12721366]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPARC antibody used in Western Blot (WB). GTX133747

GTX133747 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SPARC antibody (GTX133747) diluted at 1:1000 and competitor's antibody (sc-25574) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747

GTX133747 IHC-P Image

SPARC antibody detects SPARC protein in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SPARC antibody (GTX133747) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SPARC antibody used in Western Blot (WB). GTX133747

GTX133747 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SPARC antibody (GTX133747) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747

GTX133747 IHC-P Image

Comparison

SPARC antibody detects SPARC protein in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Antibodies: SPARC antibody (GTX133747) diluted at 1:500, and competitor's antibody diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747

GTX133747 IHC-P Image

SPARC antibody detects SPARC protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse retina.
Green: SPARC protein stained by SPARC antibody (GTX133747) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX133747

GTX133747 IHC-P Image

SPARC antibody detects SPARC protein in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SPARC antibody (GTX133747) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SPARC antibody used in Western Blot (WB). GTX133747

GTX133747 WB Image

The data was published in the 2022 in Clin Exp Dent Res. PMID: 35106969

REFERENCE

Application Reference

Hosiriluck N et al. Clin Exp Dent Res 2022; (Epub) The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169