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SPHK1 antibody

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107509
Anti-SPHK1 antibody used in Western Blot (WB). GTX107509
Anti-SPHK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107509

Cat. No. GTX107509

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SPHK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPHK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951989

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingosine kinase 1 , SPHK

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPHK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107509

GTX107509 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SPHK1(GTX107509) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SPHK1 antibody used in Western Blot (WB). GTX107509

GTX107509 WB Image

Sample (30μg whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX107509 diluted at 1:1000

Anti-SPHK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107509

GTX107509 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SPHK1(GTX107509) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Pilling D et al. BMC Immunol 2017; 18 (1) : 30 Monocyte differentiation and macrophage priming are regulated differentially by pentraxins and their ligands.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

SPHK1 antibody Cat. No. GTX107509

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-SPHK1 antibody used in Western Blot (WB). GTX107509
Date : Anonymous submitted on 29-Nov-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC-3-VGH cells
Amount used : 35μg
Blocking : 5% non-fat milk in TBST, 4°C, 1Hr
Primary Antibody : 1:3000, 4°C, 18Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169