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SPOCK3 antibody [N2C3]

Anti-SPOCK3 antibody [N2C3] used in Western Blot (WB). GTX119149
Anti-SPOCK3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX119149
Anti-SPOCK3 antibody [N2C3] used in Western Blot (WB). GTX119149

Cat. No. GTX119149

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SPOCK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SPOCK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617132

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3 , HSAJ1454 , TES-3 , TICN3

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

Proteoglycans, which consist of a core protein and covalently linked glycosaminoglycans, are components of the extracellular matrix. SPOCK3 encodes a member of a novel Ca(2+)-binding proteoglycan family.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SPOCK3 antibody [N2C3] used in Western Blot (WB). GTX119149

GTX119149 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX119149 diluted at 1:5000

Anti-SPOCK3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX119149

GTX119149 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using SPOCK3(GTX119149) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SPOCK3 antibody [N2C3] used in Western Blot (WB). GTX119149

GTX119149 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX119149 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169