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SPR antibody [3A9]

KO/KD Overexpression
Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574
Anti-SPR antibody [3A9] used in Flow cytometry (FACS). GTX83574
Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574
Anti-SPR antibody [3A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83574
Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574

Cat. No. GTX83574

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A9

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all SPR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SPR (NP_003115) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sepiapterin reductase , SDR38C1

Cellular Localization

Cytoplasm

Background

Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.

Database

Research Area

DATA IMAGES

Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574

GTX83574 WB Image

WB analysis of various cell lines using GTX83574 SPR antibody [3A9].
Loading : 35 ug per lane

Anti-SPR antibody [3A9] used in Flow cytometry (FACS). GTX83574

GTX83574 FACS Image

FACS analysis of HEK293T cells transfected with either SPR plasmid(Red) or empty vector control plasmid(Blue) using GTX83574 SPR antibody [3A9].

Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574

GTX83574 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SPR plasmid (Right) or empty vector (Left) for 48 hrs using GTX83574 SPR antibody [3A9].
Loading : 5 ug per lane

Anti-SPR antibody [3A9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83574

GTX83574 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SPR plasmid using GTX83574 SPR antibody [3A9].

Anti-SPR antibody [3A9] used in Western Blot (WB). GTX83574

GTX83574 WB Image

KO/KD

WB analysis of wild type and SPR knock out HeLa cell lysate(10μg per lane) using GTX83574 SPR antibody [3A9].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519