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SRY antibody [1G4]

Anti-SRY antibody [1G4] used in ELISA (ELISA). GTX60628
Anti-SRY antibody [1G4] used in Flow cytometry (FACS). GTX60628
Anti-SRY antibody [1G4] used in Western Blot (WB). GTX60628

Cat. No. GTX60628

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G4

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all SRY products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% protein stabilizer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen corresponding to a region within amino acids 1 and 204 of human SRY expressed in E. Coli.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sex determining region Y , SRXX1 , SRXY1 , TDF , TDY

Cellular Localization

Nucleus speckle,Cytoplasm,Nucleus

Background

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SRY antibody [1G4] used in ELISA (ELISA). GTX60628

GTX60628 ELISA Image

ELISA analysis of antigen using GTX60628 SRY antibody [1G4].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-SRY antibody [1G4] used in Flow cytometry (FACS). GTX60628

GTX60628 FACS Image

FACS analysis of HepG2 cells using GTX60628 SRY antibody [1G4].
Green : SRY
Red : negative control

Anti-SRY antibody [1G4] used in Western Blot (WB). GTX60628

GTX60628 WB Image

WB analysis of NTERA-2 cell lysate using GTX60628 SRY antibody [1G4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409