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SSB antibody

Anti-SSB antibody used in Western Blot (WB). GTX113555
Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555
Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555
Anti-SSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113555
Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555
Anti-SSB antibody used in Western Blot (WB). GTX113555

Cat. No. GTX113555

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

JC , C2C12 , 293T

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SSB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037330

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small RNA binding exonuclease protection factor La , LARP3 , La , La/SSB

Cellular Localization

Nucleus

Background

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus (SLE; MIM 152700) (Teplova et al., 2006 [PubMed 16387655]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SSB antibody used in Western Blot (WB). GTX113555

GTX113555 WB Image

Sample (30 μg of whole cell lysate) 
  A: JC
  B: C2C12
  7.5% SDS PAGE 
  GTX113555 diluted at 1:10000
 
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555

GTX113555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SSB(GTX113555) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555

GTX113555 IHC-P Image

SSB antibody detects SSB protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SSB antibody (GTX113555) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SSB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113555

GTX113555 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SSB(GTX113555) antibody at 1:200 dilution.

Anti-SSB antibody used in IHC (Paraffin sections) (IHC-P). GTX113555

GTX113555 IHC-P Image

SSB antibody detects SSB protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SSB antibody (GTX113555) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SSB antibody used in Western Blot (WB). GTX113555

GTX113555 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
10% SDS PAGE
GTX113555 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang Q et al. Virus Res 2012; 167 (2) : 302-9 Regulation of hepatitis C virus translation initiation by iron: role of eIF3 and La protein.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169