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SSEA-4 antibody

Anti-SSEA-4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48037
Anti-SSEA-4 antibody used in Flow cytometry (FACS). GTX48037

Cat No. GTX48037

Host Mouse
Clonality Monoclonal
Clone Name MC-813-70
Isotype IgG3
Application WB, ICC/IF, FACS, IP, IHC
Reactivity Human, Mouse, Rabbit, Dog, Chicken, Monkey
Package
100 μg ($339)
APPLICATION

Application Note

For flow cytometry, we recommend using 0.1 ug per million cells. Optimal working dilutions should be determined experimentally by the end user.

Specificity/Sensitivity

SSEA-4
PROPERTIES

Form

Liquid

Buffer

PBS containing 0.05% BSA and 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human embryonal carcinoma cell line 2102Ep

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

StageSpecific Embryonic Antigen4, Stage-Specific Embryonic Antigen-4, StageSpecific Embryonic Antigen 4, SSEA4, SSEA-4, Stage-Specific Embryonic Antigen 4, SSEA 4

Cellular Localization

Cell Membrane

Background

This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ (EG) cells) and human embryonic stem (ES) cells. No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Research Area

DATA IMAGES
Anti-SSEA-4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48037

GTX48037 ICC/IF Image

Immunofluorescence analysis of H9 cells using SSEA-4 (GTX48037) antibody (green) at a dilution of 1:250.

Anti-SSEA-4 antibody used in Flow cytometry (FACS). GTX48037

GTX48037 FACS Image

Cell surface flow cytometric analysis of SSEA-4 on 2102Ep cells using 0.1 ug of GeneTex anti-SSEA-4 monoclonal antibody (green histogram represents isotype control; red represents SSEA-4 antibody).

REFERENCE
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Package List Price ($)
$ 339