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United States (US)

STAT3 antibody [C3], C-term

KO/KD
GTX104616_39953_20160303_WB_shRNA_watermark_19011506_507.jpg
GTX104616_39953_20170601_WB_KO_watermark_19011506_899.jpg
GTX104616_39953_WB_M_19011506_902.jpg
GTX104616_39953_WB_R_19011506_107.jpg
GTX104616_IFA_19011506_637.jpg
GTX104616_39953_WB_hESC_19011506_941.jpg
GTX104616_WB_19011506_291.jpg

Cat No. GTX104616

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat, Plant
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , human ESC , NIH-3T3 , BCL2 , Rat2 , OC3

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APRF antibody, FLJ20882 antibody, HIES antibody, MGC16063 antibody, STAT3 antibody, acute-phase response factor antibody, DNA-binding protein APRF antibody, signal transducer and activator of transcription 3 antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104616_39953_20160303_WB_shRNA_watermark_19011506_507.jpg

GTX104616 WB Image

KO/KD

Non-transfected (–) and transfected (+) OC3 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104616_39953_20170601_WB_KO_watermark_19011506_899.jpg

GTX104616 WB Image

KO/KD

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX104616_39953_WB_M_19011506_902.jpg

GTX104616 WB Image

STAT3 antibody [C3], C-term detects STAT3 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
STAT3 antibody [C3], C-term (GTX104616) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104616_39953_WB_R_19011506_107.jpg

GTX104616 WB Image

STAT3 antibody [C3], C-term detects STAT3 protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
STAT3 antibody [C3], C-term (GTX104616) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104616_IFA_19011506_637.jpg

GTX104616 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3(GTX104616) antibody at 1:200 dilution.

GTX104616_39953_WB_hESC_19011506_941.jpg

GTX104616 WB Image

Sample (20 µg of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX104616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104616_WB_19011506_291.jpg

GTX104616 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
7.5% SDS PAGE
GTX104616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

STAT3 antibody [C3], C-term Cat No. GTX104616

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : LAMA-84 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : TBST with 0.5% BSA, 4°C, 18Hr
Primary Antibody : 1:1000, 24°C, 2Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf