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STAT3 (phospho Tyr705) antibody

Orthogonal
Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000
Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000
Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000
Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000
Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000

Cat. No. GTX118000

Reference 20
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all STAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr705 of human STAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618482

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000

GTX118000 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000

GTX118000 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000

GTX118000 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using STAT3 (Phospho Y705)(GTX118000) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000

GTX118000 ICC/IF Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2020.PMID: 32059373

Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000

GTX118000 ICC/IF Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32059373

REFERENCE

Application Reference

DM Allam et al. Iran J Pathol 2024; 19 (1) : 89-102 Role of CTLA4 and pSTAT3 Immunostaining in Prognosis and Treatment of the Colorectal Carcinoma
BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Zhao M et al. iScience 2023; 26 (9) : 107544 IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis.
Mengmeng Zhao et al. ISCIENCE 2023; S2589-0042 (23) : 01621-8 IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting proinflammatory macrophage polarization and pyroptosis
Application : WB
Reactivity : Mouse
Di Giorgio C et al. Front Oncol 2023; 13 : 1140730 Discovery of BAR502, as potent steroidal antagonist of leukemia inhibitory factor receptor for the treatment of pancreatic adenocarcinoma.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

STAT3 (phospho Tyr705) antibody Cat. No. GTX118000

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000
Date : Anonymous submitted on 25-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 60μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : Undiluted, 4°C, 16Hr
Anti-STAT3 (phospho Tyr705) antibody used in Western Blot (WB). GTX118000
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysate
Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000
Date : Anonymous submitted on 28-Mar-2017
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : embryonic fibroblast cells
Blocking : 2% BSA in PBS, 22°C, 1Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.5% TrotonX in PBS
Primary Antibody : 1:100, 4°C, 16Hr
Anti-STAT3 (phospho Tyr705) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118000
Date : Anonymous submitted on 28-Mar-2017
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : ES cells
Blocking : 2% BSA in PBS, 22°C, 1Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 0.5% TrotonX in PBS
Primary Antibody : 1:100, 4°C, 16Hr
Submit a Review
Application Reference
DM Allam et al. Iran J Pathol 2024; 19 (1):89-102 Role of CTLA4 and pSTAT3 Immunostaining in Prognosis and Treatment of the Colorectal Carcinoma
BY Wang et al. Am J Cancer Res 2023; 13 (10):4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Zhao M et al. iScience 2023; 26 (9):107544 IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting pro-inflammatory macrophage polarization and pyroptosis.
Mengmeng Zhao et al. ISCIENCE 2023; S2589-0042 (23):01621-8 IL-30 protects against sepsis-induced myocardial dysfunction by inhibiting proinflammatory macrophage polarization and pyroptosis
Application : WB
Reactivity : Mouse
Di Giorgio C et al. Front Oncol 2023; 13:1140730 Discovery of BAR502, as potent steroidal antagonist of leukemia inhibitory factor receptor for the treatment of pancreatic adenocarcinoma.
Application : WB
Reactivity : Human
Di Giorgio C et al. Cells 2022; 11 (21) Repositioning Mifepristone as a Leukaemia Inhibitory Factor Receptor Antagonist for the Treatment of Pancreatic Adenocarcinoma.
Application : WB
Reactivity : Human
Di Giorgio C et al. Front Oncol 2022; 12:939969 Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis.
Lin ZH et al. J Ethnopharmacol 2022; 282:114636 Water extract of medicinal ink (WEMI) attenuates lipopolysaccharide-induced NO production of Raw264.7 cells via downregulating JAK2/STAT3-mediated iNOS expression.
Application : WB
Reactivity : Mouse
Lecheng Zhang et al. Cell Cycle 2021; 20 (11):1052-1066 Knockdown of hsa_circ_0134111 alleviates the symptom of osteoarthritis via sponging microRNA-224-5p.
Casta?eda-Z?rraga A et al. Thorac Cancer 2020; 11 (11):3243-3251 Human skin biomarkers relationship to response to treatment with tyrosine kinase inhibitors in advanced EGFR-mutated lung adenocarcinoma.
Szu-Yuan Wu et al. Clin Transl Med 2020; 10 (8):e252 Low molecular weight fucoidan inhibits hepatocarcinogenesis and nonalcoholic fatty liver disease in zebrafish via ASGR/STAT3/HNF4A signaling.
Application : IHC-P
Reactivity : Zebrafish
Haraguchi S et al. Int J Mol Sci 2020; 21 (4) Dynamic Changes in pStat3 are Involved in Meiotic Spindle Assembly in Mouse Oocytes.
Ogawa T et al. Am J Pathol 2020; 190 (3):577-585 Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) Regulates Epidermal Keratinization under Psoriatic Skin Inflammation.
Application : IHC-P
Reactivity : Mouse
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
El-Daly SM et al. Mol Cell Probes 2019;:101442 Differential expression of miRNAs regulating NF-庥B and STAT3 crosstalk during colitis-associated tumorigenesis.
Application : IHC-P
Reactivity : Mouse
Hsieh WY et al. PLoS One 2019; 14 (1):e0210656 Renal chymase-dependent pathway for angiotensin II formation mediated acute kidney injury in a mouse model of aristolochic acid I-induced acute nephropathy.
Application : WB
Reactivity : Mouse
Choe MH et al. Oncotarget 2015; 6 (5):2654-66 ERp57 modulates STAT3 activity in radioresistant laryngeal cancer cells and serves as a prognostic marker for laryngeal cancer.
Application : IHC-P
Reactivity : Human
Uchino Y et al. Cancer Cell Int 2015; 15:30 Granulocyte colony-stimulating factor potentiates all-trans retinoic acid-induced granulocytic differentiation in acute promyelocytic leukemia cell line HT93A.
Abkhezr M et al. Mol Pharmacol 2014; 86 (2):150-8 Angiotensin II and canonical transient receptor potential-6 activation stimulate release of a signal transducer and activator of transcription 3-activating factor from mouse podocytes.
Application : WB
Reactivity : Mouse
Iriyama N et al. Leuk Lymphoma 2014;:43108 ETV6/ARG oncoprotein confers autonomous cell growth by enhancing c-Myc expression via signal transducer and activator of transcription 5 activation in the acute promyelocytic leukemia cell line HT93A.
Application : FACS
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169