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STEP / PTPN5 antibody [C1C3]

Anti-STEP / PTPN5 antibody [C1C3] used in Western Blot (WB). GTX111209
Anti-STEP / PTPN5 antibody [C1C3] used in Western Blot (WB). GTX111209
Anti-STEP / PTPN5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111209

Cat. No. GTX111209

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all STEP / PTPN5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

Mouse brain , HepG2

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STEP / PTPN5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951539

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 5 , PTPSTEP , STEP , STEP61

Database

Research Area

DATA IMAGES

Anti-STEP / PTPN5 antibody [C1C3] used in Western Blot (WB). GTX111209

GTX111209 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX111209 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STEP / PTPN5 antibody [C1C3] used in Western Blot (WB). GTX111209

GTX111209 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111209 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STEP / PTPN5 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111209

GTX111209 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using STEP / PTPN5(GTX111209) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169