APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , MCF-7 , MDA-MB-231 , rat brain , mouse brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human STRAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10617263
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
serine/threonine kinase receptor associated protein , MAWD , PT-WD , UNRIP
Cellular Localization
Cytoplasm , Nucleus
Background
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex.
Database
Research Area
DATA IMAGES
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/templates/default/js/lightbox/images/loading.gif "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
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GTX118603 WB Image
Wild-type (WT) and STRAP knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (GTX118603) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118603 ICC/IF Image
STRAP antibody [N1C3] detects STRAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: STRAP protein stained by STRAP antibody [N1C3] (GTX118603) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX118603 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (GTX118603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118603 WB Image
STRAP antibody [N1C3] detects STRAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
STRAP antibody [N1C3] (GTX118603) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118603 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (GTX118603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118603 WB Image
STRAP antibody [N1C3] detects STRAP protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
STRAP antibody [N1C3] (GTX118603) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX118603 IHC-P Image
STRAP antibody [N1C3] detects STRAP protein at cytosol on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
STRAP antibody [N1C3] (GTX118603) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for STRAP antibody [N1C3] (GTX118603). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STRAP antibody [N1C3] (GTX118603). Be the first to share your experience with this product.
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_20190412_WB_KO_watermark_w_23060519_416.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_IFA_w_23060519_533.webp "Anti-STRAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_20160114_WB_M_brain_w_23060519_196.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_WB_w_23060519_105.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_20160114_WB_R_brain_w_23060519_641.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_WB_2_w_23060519_669.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118603](/upload/website/prouct_img/small/GTX118603/GTX118603_41059_IHC_M_w_23060519_396.webp "Anti-STRAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_20190412_WB_KO_watermark_w_23060519_416.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_IFA_w_23060519_533.webp "Anti-STRAP antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_20160114_WB_M_brain_w_23060519_196.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_WB_w_23060519_105.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_20160114_WB_R_brain_w_23060519_641.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_WB_2_w_23060519_669.webp "Anti-STRAP antibody [N1C3] used in Western Blot (WB). GTX118603")
![Anti-STRAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118603](/upload/website/prouct_img/normal/GTX118603/GTX118603_41059_IHC_M_w_23060519_396.webp "Anti-STRAP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX118603")