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SUCLG1 antibody

KO/KD
Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215
Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215
Anti-SUCLG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109215
Anti-SUCLG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109215
Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215
Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215

Cat. No. GTX109215

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SUCLG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

Rat brain , Mouse brain

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human SUCLG1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952099

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate-CoA ligase alpha subunit , GALPHA , MTDPS9 , SUCLA1

Cellular Localization

Mitochondrion

Background

This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215

GTX109215 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody (GTX109215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215

GTX109215 WB Image

SUCLG1 antibody detects SUCLG1 protein by western blot analysis.
A.50 μg rat brain lysate/extract
10% SDS-PAGE
SUCLG1 antibody (GTX109215) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109215

GTX109215 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SUCLG1(GTX109215) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SUCLG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109215

GTX109215 ICC/IF Image

SUCLG1 antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: SUCLG1 protein stained by SUCLG1 antibody (GTX109215) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215

GTX109215 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX109215 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SUCLG1 antibody used in Western Blot (WB). GTX109215

GTX109215 WB Image

The data was published in the journal Dis Model Mech in 2014. PMID: 24271779

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF, IP
Reactivity : Human
Donti TR et al. Dis Model Mech 2013; Screen for abnormal mitochondrial phenotypes in mouse ES cells identifies model for Succinyl-CoA Ligase deficiency and mtDNA depletion.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169