APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:50-1:100 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rabbit, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 115-141 amino acids from the Central region of human ATP1B2.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATPase Na+/K+ transporting subunit beta 2 , AMOG
Cellular Localization
Cell membrane; Single-pass type II membrane protein
Background
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
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GTX80450 FACS Image
FACS analysis of K562 cells using GTX80450 Sodium/Potassium ATPase beta 2 antibody, Internal.
Top histogram : negative control
Bottom histogram : K562 cells
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GTX80450 IHC-P Image
IHC-P analysis of human hepatocarcinoma using GTX80450 Sodium/Potassium ATPase beta 2 antibody, Internal.
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GTX80450 WB Image
WB analysis of K562 cells and rat liver using GTX80450 Sodium/Potassium ATPase beta 2 antibody, Internal.
Loading : 35ug per lane
Dilution : 1:1000
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REFERENCE
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REVIEW
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