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Splicing Factor 1 antibody

Anti-Splicing Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54670
Anti-Splicing Factor 1 antibody used in Western Blot (WB). GTX54670
Anti-Splicing Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54670

Cat. No. GTX54670

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Splicing Factor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 1 , BBP , D11S636 , MBBP , ZCCHC25 , ZFM1 , ZNF162

Cellular Localization

Nucleus

Background

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-Splicing Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54670

GTX54670 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX54670 Splicing Factor 1 antibody.
Dilution : 1:100

Anti-Splicing Factor 1 antibody used in Western Blot (WB). GTX54670

GTX54670 WB Image

WB analysis of HepG2 cell lysate using GTX54670 Splicing Factor 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Splicing Factor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54670

GTX54670 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX54670 Splicing Factor 1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399