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Spred3 antibody

Anti-Spred3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85375
Anti-Spred3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85375
Anti-Spred3 antibody used in Western Blot (WB). GTX85375

Cat. No. GTX85375

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Mouse Brain Tissue Lysate(GTX27934)

Product Note

This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.The immunogen is located within amino acids 140 - 190 of Spred3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty related EVH1 domain containing 3 , Eve-3 , spred-3

Cellular Localization

Membrane

Background

Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist. This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.

Database

DATA IMAGES

Anti-Spred3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85375

GTX85375 IHC-P Image

IHC-P analysis of human brain tissue using GTX85375 Spred3 antibody.
Working concentration : 20 μg/ml

Anti-Spred3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85375

GTX85375 IHC-P Image

IHC-P analysis of human brain tissue using GTX85375 Spred3 antibody.
Working concentration : 2.5 μg/ml

Anti-Spred3 antibody used in Western Blot (WB). GTX85375

GTX85375 WB Image

WB analysis of mouse brain tissue lysate using GTX85375 Spred3 antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399