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Sprouty 1 antibody, C-term

Anti-Sprouty 1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41020
Anti-Sprouty 1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41020
Anti-Sprouty 1 antibody, C-term used in Flow cytometry (FACS). GTX41020
Anti-Sprouty 1 antibody, C-term used in Western Blot (WB). GTX41020

Cat. No. GTX41020

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Sprouty 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF 10μg/ml
IHC-P 1-3μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells.

IHC-P
This product has been successfully used Mouse Brain, showing nuclear staining.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX41020-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1; NP_955359.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sprouty RTK signaling antagonist 1 , hSPRY1

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Sprouty 1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41020

GTX41020 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX41020 Sprouty 1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Sprouty 1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX41020

GTX41020 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX41020 Sprouty 1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-Sprouty 1 antibody, C-term used in Flow cytometry (FACS). GTX41020

GTX41020 FACS Image

FACS analysis of PFA fixed HEK293 cells using GTX41020 Sprouty 1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-Sprouty 1 antibody, C-term used in Western Blot (WB). GTX41020

GTX41020 WB Image

WB analysis of human kidney lysate using GTX41020 Sprouty 1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399