GeneTex
United States (US)

Streptavidin protein (HRP)

Streptavidin-protein-HRP__GTX27403-1_18121411_799.jpg
Streptavidin-protein-HRP__GTX27403-1_18121411_655.gif
Streptavidin-protein-HRP_Western-blot_GTX27403-1_18121411_192.jpg

Cat No. GTX27403

Application WB, IHC-P, IHC-Fr, Dot, ELISA, IM
PRODUCT

Product Description

Streptavidin protein ( HRP )
APPLICATION

Application Note

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2

Storage

Store vial at 4ºC prior to restoration. For extended storage aliquot contents and freeze at -20ºC or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Purification


This product was prepared from chromatographically purified streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Streptavidin-protein-HRP__GTX27403-1_18121411_799.jpg

GTX27403 ELISA Image

Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)

Streptavidin-protein-HRP__GTX27403-1_18121411_655.gif

GTX27403 WB Image

Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)

Streptavidin-protein-HRP_Western-blot_GTX27403-1_18121411_192.jpg

GTX27403 WB Image

Western Blot of Peroxidase Conjugated Streptavidin. Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody (GTX27403) at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none

REFERENCE
Package List Price ($)
$ 149