Recommended Starting Dilutions:
For ICC/IF: Use at 1:10-1:50
For WB: Use at 1:10-1:50
For IHC: Use at 1:10-1:50
For ELISA: Use at 1:20-1:200
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.
Clone 0431 immunoprecipitates purified group A carbohydrate from both hot HCl & nitrous acid extracts. Works well HRP & gold conjugated. This monoclonal functions as a capture antibody on nitrocellulose but not on polystyrene solid phases. Clone 0721 immunoprecipitates purified group B carbohydrate from both autoclaved and enzyme prepared extracts but not HCl extracts.