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Suppressor of Fused antibody

Anti-Suppressor of Fused antibody used in IHC (Paraffin sections) (IHC-P). GTX33530
Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX33530
Anti-Suppressor of Fused antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33530

Cat. No. GTX33530

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Suppressor of Fused products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUFU negative regulator of hedgehog signaling , JBTS32 , PRO1280 , SUFUH , SUFUXL

Cellular Localization

Cytoplasm,Nucleus

Background

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Suppressor of Fused antibody used in IHC (Paraffin sections) (IHC-P). GTX33530

GTX33530 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33530 Suppressor of Fused antibody.
Dilution : 1:100

Anti-Suppressor of Fused antibody used in Western Blot (WB). GTX33530

GTX33530 WB Image

WB analysis of various sample lysates using GTX33530 Suppressor of Fused antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Suppressor of Fused antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33530

GTX33530 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX33530 Suppressor of Fused antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399