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TAS1R1 antibody [N1N2], N-term

Anti-TAS1R1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116068
Anti-TAS1R1 antibody [N1N2], N-term used in Western Blot (WB). GTX116068
Anti-TAS1R1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116068

Cat. No. GTX116068

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TAS1R1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human TAS1R1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621664

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

taste 1 receptor member 1 , GM148 , GPR70 , T1R1 , TR1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a G protein-coupled receptor and is a component of the heterodimeric amino acid taste receptor T1R1+3. The T1R1+3 receptor responds to L-amino acids but not to D-enantiomers or other compounds. Most amino acids that are perceived as sweet activate T1R1+3, and this activation is strictly dependent on an intact T1R1+3 heterodimer. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TAS1R1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX116068

GTX116068 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using T1R1(GTX116068) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TAS1R1 antibody [N1N2], N-term used in Western Blot (WB). GTX116068

GTX116068 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
7.5% SDS PAGE
T1R1 antibody
GTX116068 diluted at 1:1000

Anti-TAS1R1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116068

GTX116068 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using TAS1R1(GTX116068) antibody at 1:200 dilution.

REFERENCE

Application Reference

Meyer D et al. PLoS One 2012; 7 (2) : e32354 Expression of Tas1 taste receptors in mammalian spermatozoa: functional role of Tas1r1 in regulating basal Ca2+ and cAMP concentrations in spermatozoa.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169