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TBCC antibody [N1C3]

Anti-TBCC antibody [N1C3] used in Western Blot (WB). GTX105962
Anti-TBCC antibody [N1C3] used in Western Blot (WB). GTX105962
Anti-TBCC antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105962

Cat. No. GTX105962

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TBCC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

A431 , H1299 , NIH-3T3

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBCC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952147

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin folding cofactor C , CFC

Background

Cofactor C is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TBCC antibody [N1C3] used in Western Blot (WB). GTX105962

GTX105962 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX105962 diluted at 1:2000

Anti-TBCC antibody [N1C3] used in Western Blot (WB). GTX105962

GTX105962 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:H1299
12% SDS PAGE
GTX105962 diluted at 1:1500

Anti-TBCC antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105962

GTX105962 IHC-P Image

TBCC antibody [N1C3] detects TBCC protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TBCC antibody [N1C3] (GTX105962) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Knight LM et al. J Virol 2015; 89 (1) : 35-47 Merkel cell polyomavirus small T antigen mediates microtubule destabilization to promote cell motility and migration.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169