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TBCK antibody [C1C2], Internal

Anti-TBCK antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111742
Anti-TBCK antibody [C1C2], Internal used in Western Blot (WB). GTX111742
Anti-TBCK antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111742
Anti-TBCK antibody [C1C2], Internal used in Western Blot (WB). GTX111742

Cat. No. GTX111742

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TBCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , HepG2 , Molt-4

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBCK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950914

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TBC1 domain containing kinase , HSPC302 , IHPRF3 , TBCKL

Database

Research Area

DATA IMAGES

Anti-TBCK antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111742

GTX111742 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBCK(GTX111742) antibody at 1:200 dilution.

Anti-TBCK antibody [C1C2], Internal used in Western Blot (WB). GTX111742

GTX111742 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX111742 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBCK antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX111742

GTX111742 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TBCK(GTX111742) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TBCK antibody [C1C2], Internal used in Western Blot (WB). GTX111742

GTX111742 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX111742 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169