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TBRG4 antibody

Anti-TBRG4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107336
Anti-TBRG4 antibody used in Western Blot (WB). GTX107336
Anti-TBRG4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107336

Cat. No. GTX107336

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TBRG4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A431 , HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBRG4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor beta regulator 4 , CPR2 , FASTKD4

Background

May play a role in cell cycle progression.

Database

Research Area

DATA IMAGES

Anti-TBRG4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107336

GTX107336 ICC/IF Image

TBRG4 antibody detects TBRG4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TBRG4 protein stained by TBRG4 antibody (GTX107336) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-TBRG4 antibody used in Western Blot (WB). GTX107336

GTX107336 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX107336 diluted at 1:1000

Anti-TBRG4 antibody used in IHC (Paraffin sections) (IHC-P). GTX107336

GTX107336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TBRG4(GTX107336) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169