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TCF3 antibody, Internal

Anti-TCF3 antibody, Internal used in Western Blot (WB). GTX89171
Anti-TCF3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89171

Cat No. GTX89171

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Kidney shows strong nuclear staining in DCT cells.

Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89171-PEP).

Product Note

This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KAPRARTSPDEDED, from the internal region of the protein sequence according to NP_003191.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75

Cellular Localization

Nucleus

Background

This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-TCF3 antibody, Internal used in Western Blot (WB). GTX89171

GTX89171 WB Image

WB analysis of Daudi cell lysate using GTX89171 TCF3 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-TCF3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89171

GTX89171 IHC-P Image

IHC-P analysis of human kidney using GTX89171 TCF3 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf