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TCF3 antibody

Anti-TCF3 antibody used in Immunoprecipitation (IP). GTX109885
Anti-TCF3 antibody used in Western Blot (WB). GTX109885
Anti-TCF3 antibody used in Western Blot (WB). GTX109885

Cat No. GTX109885

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , NCI-H929 , BCL-1

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172469

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75

Cellular Localization

Nucleus

Background

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TCF3 antibody used in Immunoprecipitation (IP). GTX109885

GTX109885 IP Image

TCF3 antibody immunoprecipitates TCF3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 35 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 antibody (GTX109885)
7.5 % SDS-PAGE
The immunoprecipitated TCF3 protein was detected by TCF3 antibody (GTX109885) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-TCF3 antibody used in Western Blot (WB). GTX109885

GTX109885 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: NCI-H929
7.5% SDS PAGE
GTX109885 diluted at 1:5000

Anti-TCF3 antibody used in Western Blot (WB). GTX109885

GTX109885 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX109885 diluted at 1:2000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf