GeneTex
  • Country / Location Selection

United States (US)

TCF3 antibody

Anti-TCF3 antibody used in Western Blot (WB). GTX129237
Anti-TCF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129237
Anti-TCF3 antibody used in Immunoprecipitation (IP). GTX129237
Anti-TCF3 antibody used in Western Blot (WB). GTX129237

Cat No. GTX129237

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TCF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75

Cellular Localization

Nucleus

Background

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TCF3 antibody used in Western Blot (WB). GTX129237

GTX129237 WB Image

TCF3 antibody detects TCF3 protein by western blot analysis.
A. 30 μg NCI-H929 whole cell extract
B. 30 μg NCI-H929 nuclear extract
7.5 % SDS-PAGE
TCF3 antibody (GTX129237) dilution: 1:3000

Anti-TCF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129237

GTX129237 ICC/IF Image

TCF3 antibody detects TCF3 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 protein stained by TCF3 antibody (GTX129237) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TCF3 antibody used in Immunoprecipitation (IP). GTX129237

GTX129237 IP Image

TCF3 antibody immunoprecipitates TCF3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 50 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 antibody (GTX129237)
7.5 % SDS-PAGE
The immunoprecipitated TCF3 protein was detected by TCF3 antibody (GTX129237) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-TCF3 antibody used in Western Blot (WB). GTX129237

GTX129237 WB Image

TCF3 antibody detects TCF3 protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Raji whole cell extract
C. 30 μg NCI-H929 whole cell extract
7.5 % SDS-PAGE
TCF3 antibody (GTX129237) dilution: 1:2500

REFERENCE

There are currently no references for TCF3 antibody (GTX129237). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TCF3 antibody (GTX129237). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf