GeneTex
United States (US)

TCF3 antibody

GTX31402_2600_WB_20180221_18121410_454.jpg
GTX31402_1009_IHC-P_20180221_18121410_510.jpg
GTX31402_1010_IHC-P_20180221_18121410_973.jpg

Cat No. GTX31402

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TCF3 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3.The immunogen is located within amino acids 150 - 200 of TCF3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transcription Factor 3,Agm8,E2A,E47,Itf1,Tcf-3,Vdir,Bhlhb21,P75,Tcf3

Background

This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of c

Database

Research Area

DATA IMAGES
GTX31402_2600_WB_20180221_18121410_454.jpg

GTX31402 WB Image

WB analysis of human brain tissue lysate in (A) the absence and (B) presence of blocking peptide using GTX31402 TCF3 antibody.
Working concentration : 1 μg/ml

GTX31402_1009_IHC-P_20180221_18121410_510.jpg

GTX31402 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31402 TCF3 antibody.
Working concentration : 20 μg/ml

GTX31402_1010_IHC-P_20180221_18121410_973.jpg

GTX31402 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31402 TCF3 antibody.
Working concentration : 5 μg/ml