APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
EL4 Cell Lysate(GTX27183)
Product Note
At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.The immunogen is located within amino acids 70 - 120 of TCIRG1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 , ATP6N1C , ATP6V0A3 , Atp6i , OC-116kDa , OC116 , OPTB1 , Stv1 , TIRC7 , Vph1 , a3
Cellular Localization
Membrane
Background
Through alternate splicing, this gene encodes two proteins with similarity to subunits of the vacuolar ATPase (V-ATPase) but the encoded proteins seem to have different functions. V-ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX32087 WB Image
WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32087 TCIRG1 antibody. Working concentration : 0.5 μg/ml
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REFERENCE
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REVIEW
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